The Transmission
Electron Microscope
The transmission electron
microscope (TEM) operates on the same basic
principles as the light microscope but uses electrons
instead of light. What you can see with a light
microscope is limited by the wavelength of light.
TEMs use electrons as "light source" and their much
lower wavelength makes it possible to get a
resolution a thousand times better than with a light
microscope.
You can see objects to the
order of a few angstrom (10-10 m). For
example, you can study small details in the cell or
different materials down to near atomic levels. The
possibility for high magnifications has made the TEM
a valuable tool in both medical, biological and
materials research.
Magnetic Lenses
Guide the Electrons A "light
source" at the top of the microscope emits the
electrons that travel through vacuum in the column of
the microscope. Instead of glass lenses focusing the
light in the light microscope, the TEM uses
electromagnetic lenses to focus the electrons into a
very thin beam. The electron beam then travels
through the specimen you want to study. Depending on
the density of the material present, some of the
electrons are scattered and disappear from the beam.
At the bottom of the microscope the unscattered
electrons hit a fluorescent screen, which gives rise
to a "shadow image" of the specimen with its
different parts displayed in varied darkness
according to their density. The image can be studied
directly by the operator or photographed with a
camera.
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