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<qq>
	<q>
		<t goto="3">
			<q>Which one of these three photographs was taken through a phase contrast microscope?</q>
			<a1/>
			<a2>fluorescence</a2>
			<a3>transmission electron</a3>
			<c>It is image XXX. The light halo around the cell in the center of this image reveals that it was taken through a phase contrast microscope. Image YYY was taken through a AAA microscope and image ZZZ was taken through a BBB microscope.</c>
		</t>
	</q>
	<q>
		<t goto="3">
			<q>Which one of these three photographs was taken through a fluorescence microscope?</q>
			<a1/>
			<a2>phase contrast</a2>
			<a3>scanning tunneling</a3>
			<c>It is image XXX. The two distinct colors of blue and green is the result of using two different fluorescing chemicals. Image YYY was taken through a AAA microscope and image ZZZ was taken through a BBB microscope.</c>
		</t>
	</q>
	<q>
		<t goto="3">
			<q>Which one of these three photographs was taken through a transmission electron microscope?</q>
			<a1/>
			<a2>scanning tunneling</a2>
			<a3>phase contrast</a3>
			<c>It is image XXX. Image YYY was taken through a AAA microscope and image ZZZ was taken through a BBB microscope.</c>
		</t>
	</q>
	<q>
		<t goto="3">
			<q>Which one of these three images was rendered with the help of a scanning tunneling microscope?</q>
			<a1/>
			<a2>fluorescence</a2>
			<a3>phase contrast</a3>
			<c>It is image XXX. Image YYY was taken through a AAA microscope and image ZZZ was taken through a BBB microscope.</c>
		</t>
	</q>
	<q>
		<t goto="2">
			<q>With the microscope, one could study objects smaller than the naked eye could see. Who was first to describe bacteria?</q>
			<a1>Anton van Leeuwenhoek</a1>
			<a2>Zacharias Janssen</a2>
			<a3>Ernst Abbe</a3>
			<c>Anton van Leeuwenhoek used a single lens microscope to study all sorts of objects like insects and blood. In 1675 he described cells and bacteria for the first time in history.</c>
		</t>
		<t goto="2">
			<q>At the end of the 19th century, a country doctor using a microscope discovered the bacilli that caused tuberculosis. What was his name?</q>
			<a1>Robert Koch</a1>
			<a2>Filippo Pacini</a2>
			<a3>Edward B. Lewis</a3>
			<c>In 1882, the German physician Robert Koch presented his discovery of the bacteria "Mycobacterium Tuberculosis." For this achievement, Robert Koch was awarded the 1905 Nobel Prize in Medicine or Physiology.</c>
		</t>
		<t goto="2">
			<q>During which century was the microscope invented?</q>
			<a1>The 16th century</a1>
			<a2>The 18th century</a2>
			<a3>The 20th century</a3>
			<c>During the 14th century, the art of grinding lenses was developed in Italy. But it was not until the end of the 16th century that the Dutch lens grinders Hans and Zacharias Janssen made the first microscope.</c>
		</t>
	</q>
	<q>
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			<q>In which year was the Nobel Prize in physics given for the invention of the phase contrast microscope?</q>
			<a1>1953</a1>
			<a2>1972</a2>
			<a3>1943</a3>
			<c>In 1953, Frits Zernike was awarded the Nobel Prize in physics for his demonstration of the phase contrast method and especially for the development of the phase contrast microscope.</c>
		</t>
		<t goto="2">
			<q>Who invented the phase contrast microscope?</q>
			<a1>Frits Zernike</a1>
			<a2>Ernst Ruska</a2>
			<a3>Heinrich Rohrer</a3>
			<c>It was the Dutch physicist Frits Zernike who invented the phase contrast microscope in 1930. For this achievement he was awarded the Nobel Prize in Physics in 1953.</c>
		</t>
		<t goto="2">
			<q>Who designed the first transmission electron microscope?</q>
			<a1>Ernst Ruska</a1>
			<a2>Frits Zernike</a2>
			<a3>Heinrich Rohrer</a3>
			<c>It was the German physicist Ernst Ruska. He managed to solve several electron optical problems and then built the first electron microscope together with Dr. Max Knoll in 1931. For this achievement he was awarded the Nobel Prize in Physics in 1986.</c>
		</t>
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			<q>In which year did Richard Zsigmondy develop the ultramicroscope in collaboration with Heinrich Siedentopf?</q>
			<a1>1903</a1>
			<a2>1818</a2>
			<a3>1962</a3>
			<c>It was in 1903. Richard Zsigmondy was awarded the 1925 Nobel Prize in Chemistry for his work on colloid solutions and for the methods that he had developed for his studies. These methods included the use of the ultramicroscope.</c>
		</t>
	</q>
	<q>
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			<q>In a phase contrast microscope there is a phase plate. What does the phase plate do to the light that has passed through the specimen?</q>
			<a1>It increases phase differences</a1>
			<a2>It decreases phase differences</a2>
			<a3>It removes phase differences</a3>
			<c>The phase plate increases the phase difference to half a wavelength. When projected together with unaltered light, destructive interference increases the contrast.</c>
		</t>
	</q>
	<q>
		<t goto="2">
			<q>Which type of light microscope is widely used to study transparent and colorless objects?</q>
			<a1>Phase contrast microscopy</a1>
			<a2>Ultra microscopy</a2>
			<a3>Fluorescence microscopy</a3>
			<c>The phase contrast method is most popular since it can visualize objects that would be transparent in a normal light microscope.</c>
		</t>
	</q>
	<q>
		<t goto="2">
			<q>If you were to build your own phase contrast microscope, in which order would you place the phase plate, the light source and the specimen to get the best result?</q>
			<a1>light source - specimen - phase plate</a1>
			<a2>phase plate - light source – specimen</a2>
			<a3>light source - phase plate - specimen</a3>
			<c>The correct answer is: light source – specimen – phase plate. This is because the light has to pass the specimen before it reaches the phase plate.</c>
		</t>
	</q>
	<q>
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			<q>In fluorescence microscopy one uses fluorescing chemicals which emit light when exposed to radiation of a certain wavelength. How would you best describe the emitted light when you compare it to the exciting light?</q>
			<a1>It has lower energy</a1>
			<a2>It has higher energy</a2>
			<a3>It has the same energy</a3>
			<c>The emitted light must always have lower energy than the exciting light according to Stokes law, first formulated in 1852. Although the difference in energy between the emitted light and the exciting light may vary.</c>
		</t>
	</q>
	<q>
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			<q>Light is a fundamental component of most microscopes. What is light made of?</q>
			<a1>Photons</a1>
			<a2>Electrons</a2>
			<a3>Neutrons</a3>
			<c>Light is made of photons. Photons are usually described as quantum of electromagnetic radiation. They show properties of both waves and particles at the same time.</c>
		</t>
	</q>
	<q>
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			<q>When you excite a fluorescing material with exciting light of a matching wavelength, the atoms of the fluorescing material absorbs energy. Electrons inside the atoms get excited to a new energy level. What happens when the electrons relax to their ground state?</q>
			<a1>They emit photons</a1>
			<a2>They emit alpha particles</a2>
			<a3>They emit beta particles</a3>
			<c>The electrons that relax to their ground state emit one photon each. The photon is the elementary particle of light. Alpha and beta particles are subatomic particles that are radiated during radioactive decay.</c>
		</t>
	</q>
	<q>
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			<q>Emitting light from a fluorescing material has lower energy than the radiating light. What characterizes light with low energy?</q>
			<a1>It has long wavelength</a1>
			<a2>It has short wavelength</a2>
			<a3>It has random wavelength</a3>
			<c>Light of low energy has long wavelength. The wavelength of light is inversely related to its energy.</c>
		</t>
	</q>
	<q>
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			<q>How does the wavelength of an electron compare to the wavelength of a photon?</q>
			<a1>It is much shorter</a1>
			<a2>It is much longer</a2>
			<a3>It is the same</a3>
			<c>The wavelength of an electron is much shorter than the wavelength of a photon. This is why you can get much higher resolution with transmission electron microscopes than with light microscopes.</c>
		</t>
		<t goto="2">
			<q>An electron microscope operates on the same principles as a light microscope but you use electrons instead of light. Why?</q>
			<a1>You get better resolution</a1>
			<a2>You get better contrast</a2>
			<a3>You may observe living tissue</a3>
			<c>You can obtain over a thousand times higher resolution when you use a transmission electron microscope compared to a light microscope.</c>
		</t>
	</q>
	<q>
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			<q>In a normal light microscope glass lenses are used to focus the light. What is used to focus the electrons in a transmission electron microscope?</q>
			<a1>Electromagnetic lenses</a1>
			<a2>Vacuum</a2>
			<a3>Filters</a3>
			<c>In 1926, Hans Busch discovered that magnetic fields could act as lenses by causing electron beams to converge to a focus. The electromagnetic lenses inside a transmission electron microscope work according to this principle.</c>
		</t>
	</q>
	<q>
		<t goto="2">
			<q>What can you study with a light microscope?</q>
			<a1>A cell</a1>
			<a2>A macro molecule</a2>
			<a3>A virus</a3>
			<c>You can study cells with a light microscope. Macro molecules and viruses are too small to see without an electron microscope.</c>
		</t>
		<t goto="2">
			<q>Which of the following types of microscopes can reach subatomic resolutions?</q>
			<a1>The scanning tunneling microscope</a1>
			<a2>The phase contrast microscope</a2>
			<a3>The ultramicroscope</a3>
			<c>Of the three microscopes mentioned, it is only the scanning tunneling microscope that can reach subatomic resolution.</c>
		</t>
	</q>
	<q>
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			<q>The human eye has a resolution in the order of 0.1 mm which is about...</q>
			<a1>The thickness of a hair</a1>
			<a2>The size of a cell</a2>
			<a3>The thickness of bacterium</a3>
			<c>To see something smaller than the thickness of a hair you need a microscope.</c>
		</t>
	</q>
	<q>
		<t goto="2">
			<q>With a phase contrast microscope, scientists have studied...</q>
			<a1>Cell division</a1>
			<a2>Antibodies</a2>
			<a3>DNA molecules</a3>
			<c>Cell division is an example of a process that has been examined in detail with the phase contrast microscope.</c>
		</t>
	</q>
	<q>
		<t goto="2">
			<q>What is used as a "light source" in a transmission electron microscope?</q>
			<a1>Electrons</a1>
			<a2>A lightbulb</a2>
			<a3>The sun</a3>
			<c>The transmission electron microscope (TEM) operates on the same basic principles as the light microscope but uses electrons instead of light.</c>
		</t>
	</q>
	<q>
		<t goto="2">
			<q>Which microscope can be used to get three-dimensional images of a sample?</q>
			<a1>The scanning tunneling microscope</a1>
			<a2>The fluorescence microscope</a2>
			<a3>The transmission electron microscope</a3>
			<c>The scanning tunneling microscope (STM) is a type of microscope that shows three-dimensional images of a sample.</c>
		</t>
	</q>
</qq>
